Phorylation of c-Jun was determined by western blot using an anti-phospho-c-Jun (p-c-Jun) antibody. (D) Cells were transfected with a c-Jun shRNA plasmid for 48 h, and incubated with LTA for 24 h. The cell lysates were assayed by western blot using an anti-c-Jun antibody and anti-GAPDH antibody as a control (upper panel). Conditioned media (CM) and cell lysates were analyzed by zymographic analysi